Journal: Cell chemical biology

Article Title: Quantitative Interactome Proteomics Reveals a Molecular Basis for ATF6-Dependent Regulation of a Destabilized Amyloidogenic Protein

doi: 10.1016/j.chembiol.2019.04.001

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: PDIA3 (ERp57) (rabbit polyclonal G117) , CellSignaling , Cat#2881S.

Techniques: Virus, Recombinant, Sequencing, Modification, Purification, Labeling, Multiplex sample analysis, Expressing, Plasmid Preparation, Cloning, Software

Journal: Cellular Logistics

Article Title: Interaction with the effector dynamin-related protein 1 (Drp1) is an ancient function of Rab32 subfamily proteins

doi: 10.4161/21592799.2014.986399

Figure Lengend Snippet: Localization of Rab32 Family Proteins to the ER, Mitochondria and Lysosomes. A-C. HeLa cells were processed for confocal immunofluorescence microscopy for Rab32 family proteins and the ER (detected with ERp57, ( A ), mitochondria (visualized with Mitotracker, ( B ) and lysosomes (stained with LAMP-1, ( C ). Yellow color in the overlap indicates apposition of the Rab and ER or mitochondria staining. Pictures correspond to extended focus images. Scale bar = 25 μm. Representative images are shown. ( D). Co-localization was quantified using the Manders coefficient for the localization of Rabs with the ER, mitochondria and lysosomes, as shown by representative images in panels A-C. P a = 0.0011, P b = 0.0064, P c = 0.0001, P d = 0.0004. (Standard deviation is shown, n = 6) (E). Rab32 family distribution between microsomes (containing ER, endosomes and lysosomes), mitochondria and the MAM. HeLa homogenates were fractionated via the Percoll fractionation protocol into cytosol, microsomes, pure mitochondria, and MAM, as indicated on the right side. Loading was as follows: Homogenate 0.6% of total, cytosol, microsomes, pure mitochondria, MAM (all 2.4% of total).

Article Snippet: They have been purchased as follows: rabbit anti-Rab29, rabbit anti-Rab32 (Sigma Prestige Antibodies, St. Louis, MO), rabbit anti-Rab38 (abcam, Cambridge UK ab155956, used for IF), mouse anti-Rab38 (Abnova, Taipei, Taiwan, used for Western blot), rabbit anti-PKA RII, rabbit anti-LAMP1 (Santa Cruz Biotechnology, Dallas, TX), mouse anti-ERp57 (StressMarq, Victoria, BC), rabbit anti-Drp1 (Cell Signaling, Danvers, MA), rabbit anti-Syntaxin 7 (ProteinTech, Chicago, IL), mouse and rabbit anti-FLAG (Rockland, Gilbertsville, PA; Sigma, Oakville, ON), goat anti-FACL4, rabbit anti-Rab7, mouse anti-complex 2 (abcam, Cambridge, UK), mouse anti-PDI, (Thermo-Pierce, Rockford, IL), rabbit anti-calnexin antibody (our lab ).

Techniques: Immunofluorescence, Microscopy, Staining, Standard Deviation, Fractionation

Journal: eLife

Article Title: Co-movement of astral microtubules, organelles and F-actin by dynein and actomyosin forces in frog egg cytoplasm

doi: 10.7554/eLife.60047

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-PDIA3 (Rabbit polyclonal) , Boster Bio , Cat#: PB9772 , IHC (1:1000).

Techniques: Labeling, Recombinant, Expressing, Software

Journal: Marine Drugs

Article Title: Proteomic Analysis of Anti-Tumor Effects of 11-Dehydrosinulariolide on CAL-27 Cells

doi: 10.3390/md9071254

Figure Lengend Snippet: Western blotting analysis of PDIA3, Hsp60, UQCRC1, GRP78 and prohibitin of CAL-27 cells treated with increasing concentrations of 11-dehydrosinulariolide for 24 h. GAPDH was used as the internal control.

Article Snippet: The membranes were then incubated with rabbit polyclonal antibodies against human GRP-78, Hsp60, PDIA3, UQCRC1 and GAPDH (ProteinTech Group, USA) and rabbit monoclonal antibody anti human prohibitin(Epitomics, California, USA) at 4 °C for 2 h. The membranes were washed three times in PBST (10 mM NaH 2 PO 4 , 130 mM NaCl, 0.05% Tween 20), then probed with the second Ab (goat anti-rabbit and horseradish peroxidase conjugate, 1:5000 in blocking solution) for 1 h. After it was washed with PBST for three times, the enzyme activity on the blot was visualized through chemiluminesence by adding ECL Western Blotting Reagents (Pierce).

Techniques: Western Blot, Control